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e coli bl21 de3  (GE Healthcare)
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k1612 1 e coli bl21 de3 plyss thermo fisher scientific cat c606003  (Thermo Fisher)
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hsdr17 reca1 enda1 gyra96 thi 1 rela1 invitrogen bl21  (Thermo Fisher)
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e. coli bl21 (de3) strain  (Boehringer Ingelheim)
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pokd4-smac-flag  (SMAC Corp)
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escherichia coli bl21 star (de3  (Thermo Fisher)
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bl21(de3)-condon1ril  (Thermo Fisher)
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bl21(de3) overexpression strain  (Thermo Fisher)
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Thermo Fisher bl21(de3) overexpression strain
Strains and plasmids used in this study
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gst c2 domain fusion proteins e coli bl21 de3 cells  (GE Healthcare)
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GE Healthcare gst c2 domain fusion proteins e coli bl21 de3 cells
Strains and plasmids used in this study
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benzonase nuclease  (Millipore)
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Millipore benzonase nuclease
Strains and plasmids used in this study
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e. coli bl21 gold(de3)plyss  (Agilent technologies)
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Agilent technologies e. coli bl21 gold(de3)plyss
Strains and plasmids used in this study
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Strains and plasmids used in this study

Journal: Applied and Environmental Microbiology

Article Title: Thiaminase I Provides a Growth Advantage by Salvaging Precursors from Environmental Thiamine and Its Analogs in Burkholderia thailandensis

doi: 10.1128/AEM.01268-18

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: Due to the potential for thiamine adsorption to glassware ( 60 ), we took precautions to ensure no contamination of DM4 occurred using our published glassware washing protocol ( 61 ). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Strain or plasmid Genotype a Source or reference Organism strains E. coli DH5α Invitrogen BL21(DE3) Overexpression strain Invitrogen B. thailandensis E264 Wild type 67 BT10432 E264 thiA ::ISlacZ-PrhaBo-Tp/FRT 63 BT10432- thiC BT10432 Δ thiC :: tetR This study BT10432- thiG BT10432 Δ thiG :: cat This study BT10432- thiCthiG BT10432 Δ thiC :: tetR Δ thiG :: cat This study E264- thiC E264 Δ thiC :: tetR This study E264- thiG E264 Δ thiG :: cat This study E264- thiCthiG E264 Δ thiC :: tetR Δ thiG :: cat This study S. cerevisiae InvSc1 MAT a / MAT α leu2/leu2 trp1-289 / trp1-289 ura3-52 / ura3-52 his3 -Δ 1 / his3 -Δ 1 62 Plasmids pMQ87 CEN6 ARSH4 aaC1 lacZa oriT ori ColE1; Gm r 62 pMQ87 thiC pMQ87 Δ thiC :: tetR This study pMQ87 thiG pMQ87 Δ thiG :: cat This study pET-28 bcmE Overexpression vector containing bcmE ( thiA Cb ) 25 Open in a separate window a Gm r , gentamicin resistance.

Techniques: Plasmid Preparation, Over Expression